Oxidative protein labeling in mass-spectrometry-based proteomics

Oxidative protein labeling in mass-spectrometry-based proteomics

Oxidation of proteins and peptides is a common phenomenon, and can be employed as a labeling technique for mass-spectrometry-based proteomics. Nonspecific oxidative labeling methods can modify almost any amino acid residue in a protein or only surface-exposed regions. Specific agents may label reactive functional groups in amino acids, primarily cysteine, methionine, tyrosine, and tryptophan. N...

Labeling Methods in Mass Spectrometry Based Quantitative Proteomics

Proteomics is loosely defined as the description of sets of proteins from any biological source, which have in most cases been identified by using mass spectrometry. However, only the mere identity of proteins present in a certain sample does not give any information about the dynamics of the proteome, involving relevant cellular events such as protein synthesis and degradation, or the formatio...

Computational Mass Spectrometry–Based Proteomics

Proteomics is defined as the system-wide characterization of all the proteins in an organism in terms of their sequence, localization, abundance, post-translational modifications, and biomolecular interactions. Modern proteomic investigations are increasingly quantitative and comprehensive [1]. Examples include the relative quantification of over 4,000 proteins in haploid and diploid yeast, whi...

mass spectrometry-based proteomics in the life sciences: a review

proteomics concerns itself with the characterization and function of all cellular proteins, the ultimate determinants of cellular function. mass spectrometry has emerged as the preferred method for in-depth characterization of the protein components of biological systems. using mass spectrometry, key insights into the composition, regulation and function of molecular complexes and pathways have...

Quantitative Proteomics Using iTRAQ Labeling and Mass Spectrometry